Coding

Part:BBa_K4716995:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


PEP (prolyl endopeptidase from Aspergillus oryzae RIB40)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1114


Design Notes

We use this part together with the gene of Fvp-p, our purpose is to compare the gluten 33-mer peptide digestion effect of both of them.


Source

The sequence of this part we directly take from the gene bank of NCBI, the source: https://www.ncbi.nlm.nih.gov/nuccore/XM_001825944.2?report=genbank

References